Sweet sorghum is a domesticated grass containing a sugarrich juice that can be readily utilized for ethanol production. Sorghum bicolor is a medicinal plant used in benin to treat anemia. Improved characterization of sorghum tannins using sizeexclusion chromatography r. In the united states, sorghum is primarily used for animal feed and ethanol production but has potential to provide value. Traditionally used organic solvents for these compounds extraction are toxic and are not acceptable for food industry. It can produce tillers adventitious stems originating from the plant base but not rhizomes. Firststrand cdna was synthesized with the iscript cdna synthesis kit biorad, hercules, ca. Histological observation of the effect of aqueous extract. The interest in stable natural colorants for food applications continues to grow. Histological observation of the effect of aqueous extract of. A chromosomescale assembly of the sorghum genome using. May 27, 2019 the process of crop domestication often consists of two stages. Te west african orgum bicolor leaf seat etract jobelyn and its diverse terapeutic potentials 21 copyrigt 2018 kubena et al citation. Genetic regulation of development in sorghum bicolor viii.
Moench grain conditions on occurrence of mycotoxinproducing fungi alex m. It grows in dry, sodden or high salinity soil and is a heattolerant plant considered to hold the most important rate of phenolic components. Most of the sugar is stored inside the cells of the stalk tissue and can be difficult to release, a necessary step before conventional fermentation. The bioactive composites of the stem, the leaf, the grains, the seeds. Extraction of sorghum bicolor dry stem bark leave sheath was obtained from herb sellers at ile ife, osun state, nigeria. Lignocellulosic hydrolysate of the juice extracted stalk hs. Extraction methods and food uses of a natural red colorant. Sweetstem sorghum is a multipurpose crop, yielding food in the form of grain, fuel in the form of ethanol from its stem juice, and fodder from its leaves and grain. Genetic regulation of development in sorghum bicolor. Figure 1 a brief summary of the general approaches in the extraction, isolation and characterization of bioavailable compounds in dried sorghum bicolor leaf sheath adopted from makanjuola et al, 2018. Tissue from the bmr63 mutant and the normal isoline n3, planted in the greenhouse west lafayette, in, was harvested at stage 2 and processed as. Extraction and characterization of sorghum sorghum bicolor l. We compared the ability of simple flavonoids and proanthocyanidins in sorghum bicolor bran extracts to inhibit enzymes in vitro. Selective pressure to increase sugar accumulation in certain varieties of the cereal crop sorghum bicolor is an excellent.
When combined with current sugar extraction methods, the overall ethanol production efficiency would increase compared to. Seeds were sown in pots filled with soil mixture 3. Moench fermented with aspergillus oryzae nk fsbe was used for experiments. Sorghum originated in africa, and is now cultivated widely in tropical and subtropical regions. Breakthrough technologies semiautomated feature extraction from rgb images for sorghum panicle architecture gwas1open yan zhou,a srikant srinivasan,a,2 seyed vahid mirnezami,b aaron kusmec,a qi fu,c,3 lakshmi attigala,a maria g. Sweet sorghum will thrive under drier and warmer conditions than many other crops and is grown. High molecular weight hmw genomic dna was extracted from sorghum tx430 adult leaf tissue using a. A simplified method for fractionation and analysis of. Evaluation of the antimicrobial activity of saponins extract. This study compared the colour and anthocyanin composition from traditional extraction methods to determine options for improvement and use of the red.
The main purpose of this paper it is evaluate the phenolics compounds extraction in sorgo grains using colorimetric assay. Efficient extraction method to collect sugar from sweet sorghum. Sorghum shell is a rich source of phenolic compounds. The west african sorghum bicolor leaf sheath extract jobelyn and its diverse therapeutic potentials. The combined ethanolpotash extract of sorghum bicolor leaf sheath administered to rats resulted in significant p s orghum sorghum bicolor l. Sorghum grain is the fifth most important cereal in the world. It is cultivated in different parts of the world and it has formed a part of the farming system of the people in these ecologies lukhele, 1981. Sorghum bicolor extract as a counter stain in hibiscus extract nuclear stain. Pdf extraction and characterization of sorghum sorghum bicolor. Being the fifth most important cereal grain, sorghum sorghum bicolor l. Schnablea,c,4,5 adepartment of agronomy, iowa state university, ames, iowa 50011.
Effects of aqueous extract of sorghum bicolor on hepatic, histological and haematological indices in rats. Oct 21, 2019 in the united states, sorghum is primarily used for animal feed and ethanol production but has potential to provide value. Varieties of s bicolor, known as grain sorghum, are cultivated for human food and animal feed. Moench is a crop indigenous to africa, where it appears to have been domesticated in ethiopia about 5 000 years ago. The efficacy of jobelyn sorghum bicolor extractin the. Sorghum seeds are utilized for both human and animal feed, and cultivars are processed for syrup, sugar, and alcohol. This method for purifying tannin from sorghum grain yields a very high quality procyanidin. Sorghum is the fifth most important cereal crop that is widespread in the semiarid regions of the world with an annual production of 65. Growth characteristics also vary depending on the location grown, inputs, and agronomic practices. Four local indian varieties of sorghum csh9, csh5, dadar and parbhani were subjected for proximate analysis and characterization of extracted starch. In particular, aromatase is a target for breast cancer therapy, and inhibition of. Cool and hot alkaline extraction are the best methods to extract the pigment.
High concentrations of its sugar juice can be extracted from the stem by. Pdf efficiency of sorghum bicolor extract in the treatment of. Evaluation of aqueous methanolic extract of sorghum bicolor leaf base for antinociceptive and antiinflammatory activities. Sorghum sorghum bicolor includes widely diverse types of annual and perennial types. Impact of metagenomic dna extraction procedures on the.
The histomorphological integrity of nucleus and cytoplasm if preserved by the application of the two local dyes will add to knowledge on isoelectrically compatible local dyes that are suitable for use in histological demonstration of. Extraction and characterization of sorghum sorghum. When combined with current sugar extraction methods, the overall ethanol production efficiency would increase compared to current field practices. Comparison of lipid extraction methods of foodgrade sorghum sorghum bicolor using hexane.
Pdf extraction and characterization of sorghum sorghum. Salas fernandez,a baskar ganapathysubramanian,b and patrick s. The west african sorghum bicolor leaf sheath extract jobelyn. According to the food and agriculture organization fao, the top sorghum producing countries have been the united states, nigeria, india, mexico, and argentina table. Sorghum oil dispersible powdered extract is derived from the stalks and leaves of sorghum bicolor, a tall grass, originally native to africa but now cultivated in other tropical climates.
In vitro expression of endothelial adhesion molecules vcam1 and icam1 and proinflammatory factor cyclooxygenase2 was significantly decreased and that of the antiatherogenic factor heme oxygenase1 significantly increased. Moench is a native grass species of the arid and semiarid regions of africa kimber 2000. Monech is the fifth most important cereal crop worldwide, both in terms of planted area and metric tons harvested. Introduction sorghum, which belongs to the family graminae, is the fourth most important cereal crop after wheat, rice and maize.
Pdf four local indian varieties of sorghum csh9, csh5, dadar and parbhani were subjected for proximate analysis and characterization of extracted. Introduction exposure to drugs and chemicals often induce toxicity to living organisms. The result of total phenolic contents tpc indicated that 60% ethanol extract of s. Moench stem and root tissues were extracted using two classical dna extraction protocols ctab and sdsbased and five commercial kits. This study aimed to evaluate its therapeutic efficacy in anemia treatment. Aug 02, 2010 ultrasound assisted extraction of polyphenolic compounds from red sorghum sorghum bicolor l. Sweet sorghum is any of the many varieties of sorghum which have a high sugar content. To evaluate possibility as a skin whitening agent of sorghum bicolor s. Moench, the worlds fourth major cereal in terms of production, and fifth in acreage following wheat, rice, maize and. A number of protocols have been developed for extraction of dna from fresh and dry plant leaves 29.
Evaluation of the antimicrobial activity of saponins. Phenolic acids and flavonoids in nonfermented and fermented. Efficient extraction method to collect sugar from sweet. Some compounds isolated from sorghum bicolor, such as methyl 3,4dihydroxybenzoate, 3,4,5trihydroxycinnamate, methyl 3,4dihydroxycinnamate, caffeoyl glycolic acid methyl ester and 1dcaffeoylglycerol, showed inhibitory potential on nitric oxide production and. The crop is a heat and drought tolerant, drylandadapted crop that is generally grown in marginal crop production areas.
By using buffers optimized for extraction of sorghum proteins, we hypothesized that sonication time could be drastically shortened com. A simplified method for fractionation and analysis of waxes. The west african sorghum bicolor leaf sheath extract. Antimelanogenic effect of ethanolic extract of sorghum. Moench, the worlds fourth major cereal in terms of production, and fifth in acreage following wheat, rice, maize and barley, is a staple food crop of millions of poor in. Jan 10, 20 sweet sorghum is a domesticated grass containing a sugarrich juice that can be readily utilized for ethanol production. Semiautomated feature extraction from rgb images for. Sonication and waterbath methods extraction of phenolics compounds in sorghum bicolor l. Enhanced extraction of phenolics and antioxidant capacity. Extraction and characterization of sorghum sorghum bicolor. Some compounds isolated from sorghum bicolor, such as methyl 3,4dihydroxybenzoate, 3,4,5trihydroxycinnamate, methyl 3,4dihydroxycinnamate, caffeoyl glycolic acid methyl ester and 1dcaffeoylglycerol, showed inhibitory potential on nitric oxide production and reduction of the expression of in ds. Starch was extracted from sorghum flour produced with grains cultivated in brazil.
Starch from the above varieties was extracted by steeping, wet grinding and sedimentation process. Technical report on the notification of syrup from sorghum. Improved characterization of sorghum tannins using size. Sorghum flour is rich in phytochemical components, including tannins, phenolic acids, anthocyanins, phytosterols, and policosanols, with a potential to benefit human health. This document is intended to provide background information on the biology of cultivated sorghum bicolor, its identity, geographical distribution, reproductive biology and ecology, related species, the potential for gene introgression from s. High molecular weight hmw genomic dna was extracted from sorghum tx430 adult leaf tissue using a protocol originally designed for the extraction. Moench starch article pdf available in international food research journal 191. Sorghum is a staple cereal crop that is well adapted to arid and semiarid lands asals. Rapid isolation of sorghum and other cereal starches using. In general, forage sorghums are taller plants with later maturity dates and more vegetative growth than grain sorghums. Sorghum bicolor is the fifth most cultivated cereal crop worldwide1. A red pigment extracted from the leaf sheaths of a sorghum variety sorghum bicolor with a high content of apigeninidin is widely used as a biocolorant in processed foods in west africa.
While this crop holds much promise as an arid land sugar source for biofuel production, a number of challenges must be. Certain varieties, known as sweet sorghum, concentrate sugar in the pith cells of the stalk and are cultivated for sugars and syrups. Moench ag1040 grains evaluated by colorimetric assay m. Ultrasound assisted extraction of polyphenolic compounds from red sorghum sorghum bicolor l. Sorghum extract is known in the textile industry for the range of brown hues it produces when used as a botanical dye.
Moench is widely used as food, feed, fiber, and bioenergy crop. Moench, extraction of starch, proximate analysis, characterization of sorghum starch introduction sorghum sorghum bicolor l. To investigate the suitability of alcoholic extract of sorghum bicolor stalk as a counter stain for hibiscus extract stained nuclei. This study compared the colour and anthocyanin composition from traditional extraction methods to determine options. The immunostained nitrocellulose membrane mas scanned. Impact of metagenomic dna extraction procedures on the identi. Optimization of a high throughput, cost effective, and allstage dna extraction protocol for sorghum sorghum bicolor 244 and extractions, and exportation of the seed samples to other countries is often restricted. Sorghum bicolor genotypes parbani moti, phule vasudha pvs, s35, m35, and btx623 seeds were collected from the sorghum breeding unit of the international crops research institute for the semiarid tropics icrisat in patancheru, india. Apigeninidin is the pigement that gives the colour, this has to be extracted from the sorghum plant.
A new reference genome for sorghum bicolor reveals high. Shoot growth, tillering, flowering, gib are differentially affected 3erellin biosynthesis, and phytochrome levels 3y dosage of the ma3r allele kenneth r. Starch from the above varieties was extracted by steeping, wet grinding and sedimentation. Common names used by the applicant are sorghum or sweet sorghum. Pdf comparison of lipid extraction methods of foodgrade. After juice extraction, the extracted stalks were collected, washed several times with hot water to. Nov 19, 2018 minion sequencing metrics and characterization. The water extraction method has the potential to be used for sugar extraction from both fresh sweet sorghum stalks and dried ones. Previously, we reported an optimum sorghum protein extraction condition of ph, detergent type, reducing agent type, and sampletosolvent ratio park and bean 2003. Extraction and analysis of antioxidant properties of. Wet milling sorghum to extract starch is not common, but may still be done in some countries to. Sonication and waterbath methods extraction of phenolics.
Sorghum bicolor poaceae is the oldest cultivated plant in the world. A genomewide analysis of the cinnamyl alcohol dehydrogenase. The mdna yields and quality as well as the reproducibility were compared. While this crop holds much promise as an arid land sugar source for biofuel production, a number of challenges must be overcome. Moench is an accepted scientific name and sorghum saccharatum l. I want to know about dyes extraction from sorghum plant. The plant specimen was identified and authenticated by a taxonomist at the department of botany, obafemi awolowo university, ile ife and a voucher number was given. Moench mapulakgomotsoannah maropola a, jeanbaptisteramond a,b, marlatrindade a.
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